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3rd International Conference on Applied Crystallography

Atlanta, USA

Ming-Yue Lee

Ming-Yue Lee

Arizona State University, USA

Title: Serial crystallography of a G-Protein coupled receptor using polychromatic synchrotron radiation source

Biography

Biography: Ming-Yue Lee

Abstract

Since the first successful serial crystallography (SX) experiment at synchrotron radiation sources, the popularity of this approach has continued to grow, showing that 3rdgeneration synchrotrons can be viable alternatives to scarce X-ray free electron laser sources. Synchrotron radiation flux may be increased about 100 times by a moderate increase in bandwidth (“pink beam” conditions) at some cost in data analysis complexity. Here, we report the first high-viscosity injector-based pink beam SX experiments. The structures of A2A adenosine receptor (A2AAR) and proteinase K (PK) were determined to 4.2Å and 1.8Å resolution using 24 and 4 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A2AAR required an alternative data processing strategy. This demonstration of the feasibility presents new opportunities for the time-resolved experiments with micro-crystals to study structural changes in real-time at pink beam synchrotron beamlines worldwide